《植物生理学报》 2012, 48(12): 1164-1172

超表达AhNCED1拟南芥植株在渗透胁迫下抗氧化能力和抗旱相关下游基因表达变化

李嘉怡^1,*, 苏良辰^1,2,*, 何月容¹, 李玲^1,**, 唐慧玲¹, 吴嘉莉¹

1华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广州510631; 2遵义医学院珠海校区生物工程系, 广东 珠海519041

通信作者：李嘉怡；E-mail: liling502@126.com；Tel: 020-85211378

摘　要：

利用纯系超表达AhNCED1拟南芥植株, 在添加300 mmol•L-1山梨醇的MS培养基上胁迫培养, 分析其表型及抗氧化能 力。结果表明, 在正常情况及300 mmol•L-1山梨醇处理10 d条件下, 与129B08/nced3突变体和野生型比较, 超表达AhNCED1 植株生长状况好, 主根长度增加, 幼苗体内O2·ˉ含量水平较低, POD和SOD酶活性较高。说明NCED1基因对于拟南芥体内抗 氧化酶系统的维持有重要意义。用sqRT-PCR方法检测结果表明, 野生型和超表达AhNCED1拟南芥植物体内KIN1、COR47 和RAB18基因表达随脱水延长而明显增强, 且在超表达AhNCED1拟南芥中增强幅度大。

关键词：超表达AhNCED1; 拟南芥; 渗透胁迫; 抗氧化水平; 基因表达

收稿：2012-09-17   修定：2012-11-15

资助：国家自然科学基金(30771297)和广东省自然科学基金(06025049和10251063101000010)。

Antioxidant Activity and Expression of Downstream Genes Related with Drought Resistance in Transgenic AhNCED1 Arabidopsis under Osmotic Stress

LI Jia-Yi^1,*, SU Liang-Chen^1,2,*, HE Yue-Rong¹, LI Ling^1,**, TANG Hui-Ling¹, WU Jia-Li¹

¹Guangdong Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, China; ²Department of Biological Engineering of Zunyi Medical College Zhuhai Campus, Zhuhai, Guangdong 519041, China

Corresponding author: LI Jia-Yi; E-mail: liling502@126.com; Tel: 020-85211378

Abstract:

Pure line of over-expressed AhNCED1 plants were planted on MS culture medium with 300 mmol·L^-1 sorbitol, and the antioxidant activity and their phenotype were analyzed. The results showed that, both in normal conditions and treated by 300 mmol·L^-1 sorbitol, over-expressed AhNCED1 plants were growing better than 129B08/nced3 mutant and wild type, while the main root lengths of over-expressed AhNCED1 plants were longer than them when planted 10 d. In the meanwhile, over-expressed AhNCED1 plants had lower O₂^·ˉ content level than Col-0 and 129B08/nced3 mutant, and SOD and POD activities were higher. The results showed that the NCED1 gene played an important role in maintaining the antioxidant enzymes system. It could be well studied by sqRT-PCR that the expression of KIN1, COR47 and RAB18 were increasing under the early dehydration treatment in Col-0 and the over-expressed AhNCED1 plants, while it was markedly increasing in over-expressed AhNCED1 plants.

Key words: over-expressed AhNCED1 gene; Arabidopsis; osmotic stress; antioxidant activity; gene expression